A Joint Facility of the Departments of Biology and Geology
Bates College
Lewiston, Maine, 04240

Contact:

EM Lab: (207) 786-6494 or
Greg Anderson (207)786-6110
Email: ganderso@bates.edu

This laboratory is also affiliated with:  BCCIC logo


Example images from students and staff


Faculty and Staff

 Biology  Geology Chemistry 
Robert Thomas Dyk Eusden  Matt Cote
Greg Anderson
Assistant in Instruction
EM Tech
John Creasy  
 

 

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 About the Laboratory

The Electron Microscopy and X-Ray Microanalysis Laboratory is a shared facility of the Biology and Geology Departments at Bates College. The lab is based upon an analytical, variable vacuum FE-SEM (JEOL JSM-7100FLV with a Noran System 7 EDS System) and is used by faculty and students for research, teaching, and outreach programs in Biology, Geology, and Chemistry.

For information about in-house training tutorials, demonstrations, or
outreach programming, contact:
Greg Anderson
207-786-6110
ganderso@bates.edu

 

 Our Academic Program

Two courses -one in biology and one in geology - are offered and serve as the primary mode of training students in electron microscopy. Biology teaches the Short Term course Bio s34 - Electron Microscopy, a course focused on biological applications of electron microscopy. John Creasy (Geology) teaches Geo 223 - Mineralogy, a course which incorporates x-ray microanalysis applications mineral analysis. Greg Anderson, Assistant in Instruction in Biology, overseas activities in the lab and assists in all these courses, providing much of the hands-on training, and provides outreach programming. Students and staff are also trained via small group or individual tutorials on an as need basis.

 

 

 Microscopy and Microanalysis Training

Training in the use of the SEM/EDS system is available by taking Bio 341 or Geo 223, or by arranging for a tutorial with Greg Anderson. Students are encouraged to take one of the courses, if possible. The two EM courses are generally offered in alternate years.

Our training approach grounds the student in the fundamental theory and operation of the systems so that they can think through problems of sample preparation and use the technologies to their best advantage. Our laboratory exercises focus on both theoretical and practical applications of the technologies to reinforce basic understanding of electron optical systems, beam-specimen interactions, and sample preparation. Both the biology and geology EM courses culminate in individual projects, often related to thesis or independent study problems, that encourage the student to apply and broaden their working knowledge of the technologies.

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Equipment

 SEM

 EDS

 Other Equipment

Field Emission Variable Vacuum Scanning Electron Microscope

The JEOL JSM-7100FLV scanning electron microscope (FE-SEM) was acquired in 2012. This instrument is equipped with sample chamber SE detector and two backscatter detectors: a retractable, short working distance HV backscatter detector and a LV detector. It is equipped with a standard tungsten filament gun and offers resolution of approximately 2 nm at 30 kV. We have an IR chamber-cam which allows the operator to view the sample chamber while in operation. We do not have in-the-lens imaging capability.

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 Aphid feeding on a moss of the genus Polytrichum. (photo: R.Thomas)

 

 

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Energy Dispersive Spectrometer

The SEM is equipped with a Thermo/Noran System 7 Energy Dispersive Spectrometer and Ultra-Dry detector. This system enables quantitative spectrum analysis with and without standards, spectral imaging, x-ray mapping, point and shoot, linesan, and digital image acquisition.

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Related Support Equipment and Services

    • Denton Vacuum DV1 Carbon Coater; attachments for rods or yarn (1997)
    • Anatech Hummer 6.6 Sputter Coater with Au/Pd target and stage rotation (2010)
    • Ladd Research CO2 Critical Point Dryer (1990)
    • Scandium Image processing and analysis software
    • Adobe Photoshop
    • HP 800PS Wide format printer
    • Fume hood
    • Complete machine shop and machinist

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Sources of funding and other support

We are grateful for support, both as grants and in-kind contributions to the following organizations and individuals:

    • Sherman Fairchild Foundation
    • National Science Foundation
    • Hughes Biomedical Foundation grants to Bates College
    • JEOL USA Ltd, Peabody, MA
    • Bates College

 

 

Example images from the lab


Diatom from high arctic lake sediment.

   

   

   

 
Tabitha Abrazinski 2005
This image of a freeze fractured frog gastrula is a composite of 132 individual frames that were stitched together using Adobe Photoshop. The original image is 1:1 x350 mag. Overlying images were taken at different depths to achieve greater depth of field.
 
Distribution of silica in a stem cross section of horsetail rush (Equisetum) viewed by x-ray mapping.


Laboratory for Electron Microscopy
and X-ray Microanalysis

B-10 Carnegie Science Building
44 Campus Avenue
Bates College
Lewiston, ME 04240
Lab: (207) 786-6494 or Greg Anderson: 786-6110

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Copyright 2013 Bates College | Modified on 4-30-13 gja