Bio Review Notes #24
GENETICS: CHROMOSOMES AND LINKAGE
Performance Objectives:
Chromosome types are distinguished by centromere location. Genes located on the same chromosome tend to be inherited together as a group, but these groups can be broken apart by "crossing over".

Chromosome morphology:   Eucaryotic chromosomes contain proteins (histones and nonhistones) plus DNA. Some DNA winds around clumps of histone to form nucleosomes, protecting that DNA from transcription. Chromosome arms attach to a dense region called the centromere. Chromosome types are distinguished as follows:
  • Metacentric: centromere centrally located; two long arms
  • Sub-metacentric: centromere off-center; arms a bit unequal
  • Acrocentric: centromere near one end, second arm short
  • Telocentric: centromere at one end; no second arm

Chromosomal theory of inheritance:   Genes are located on chromosomes.
  • The location of each gene is called its locus.
  • Linkage:   Genes on the same chromosome are linked (unless they are very far apart) and are inherited together as a linkage group. The number of linkage groups always equals the haploid number (N).
  • Crossing over:   Sometimes, in Prophase I of meiosis, chromosome arms may break and rejoin in switched order, rearranging linked genes.
  • Chromosome maps:   The probability of crossing over between linked genes varies with the distance between them. Recombination frequencies can thus be used to make chromosome maps (linkage maps).
  • Proof of the chromosomal theory:   Experiments show that crossing over of chromosomes always accompanies the recombination of linked genes.

Genetic crosses involving linked genes:
  • AABB x aabb is no longer the same as AAbb x aaBB
  • F1 genotypes are heterozygous at each locus. During formation of F1 gametes, parental combinations of alleles tend to stay together.
  • Each F1 individual is crossed to an organism recessive for all genes being studied (a test cross). Phenotypes in the offspring of such a cross correspond to genotypes in the F1 gametes.

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